The Catalytic Activity of Carboxypeptidase-degraded Aldolase
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چکیده
منابع مشابه
The catalytic activity of carboxypeptidase-degraded aldolase.
One approach to the general problem of the structure and function of enzymes involves proteolytic hydrolysis with examination of the structure, composition, and catalytic activity of the hydrolysis products. Fragments retaining partial enzyme activity have been obtained from trypsin and pepsin by autolysis (2, 3) and from trypsinogen by peptic digestion (4). Over half of the amino acid residues...
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heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...
15 صفحه اولComparative studies of liver and muscle aldolase. I. Effect of carboxypeptidase on catalytic activity.
The demonstration (l-3) of a distinctive pathway of fructose metabolism in liver via the aldol cleavage of fructose l-phosphate emphasized the possibilty in this tissue of an enriched catalytic system for the facilitation of this reaction since the classical fructose-l ,gdiphosphate aldolase isolated from muscle tissue exhibits relatively little activity toward fructose l-phosphate. The early e...
متن کاملComparative Studies of Liver and Muscle Aldolase. Ii. Immunochemical and Chromatographic Differentiation.
Crystalline fructose diphosphate aldolase preparations obtained from rabbit muscle and bovine liver differ in catalytic efficiency (fructose diphosphate cleavage activity of muscle aldolase is about 10 times that of the liver enzyme) and substrate specificity (the fructose diphosphate to fructose l-phosphate activity ratio is 50 for muscle and 1 for liver aldolase) (2). In spite of these distin...
متن کاملFlow cytometric screening of aldolase catalytic antibodies.
High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)69749-2